N-Terminal speciation for native chemical ligation
نویسندگان
چکیده
منابع مشابه
Specific N-terminal protein labelling: use of FMDV 3C pro protease and native chemical ligation.
We report an effective strategy for generating N-terminal cysteinyl proteins by proteolytic cleavage using the enzyme 3C pro, suitable for a wide range of applications via native chemical ligation.
متن کاملSimplifying native chemical ligation with an N-acylsulfonamide linker.
We report a simplified procedure for the chemical ligation of peptides by using the sulfamylbutyryl linker as a mildly activating group capable of participating in ligation. When the peptidyl N-methylsulfonamide is directly added with excess thiols to ligation reactions, the speed of reaction is comparable to native chemical ligation.
متن کاملSelenocysteine in native chemical ligation and expressed protein ligation.
L-Selenocysteine (Sec or U) has been called the “21st amino acid”.1 Like the twenty common amino acids, selenocysteine is inserted during the translation of mRNA and has its own tRNASec and codon, UGA. This codon also serves as the opal stop codon. Decoding a UGA codon as one for selenocysteine requires a special structure in the 3′ untranslated region of the mRNA called a selenocysteine insert...
متن کاملShifting Native Chemical Ligation into Reverse through N→S Acyl Transfer
Peptide thioester synthesis by N→S acyl transfer is being intensively explored by many research groups the world over. Reasons for this likely include the often straightforward method of precursor assembly using Fmoc-based chemistry and the fundamentally interesting acyl migration process. In this review we introduce recent advances in this exciting area and discuss, in more detail, our own eff...
متن کاملChemical Probes for Protein N-terminal Methyltransferase
CHEMICAL PROBES FOR PROTEIN N-TERMINAL METHYLTRANSFERASE. Rong Huang, Gang Zhang, Yunfei Mao, & Stacie Lynn Richardson, Department of Medicinal Chemistry, Institute for Structural Biology and Drug Discovery, Virginia Commonwealth University, Richmond VA 23219. The objective of this study is to develop chemical probes for N-terminal RCC1 methyltransferase (NRMT1). NRMT transfers methyl groups fr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Chemical Communications
سال: 2020
ISSN: 1359-7345,1364-548X
DOI: 10.1039/d0cc01604g